Back to Search Start Over

Secretin release from the isolated, vascularly perfused pig duodenum.

Authors :
Holst JJ
Lauritsen K
Jensen SL
Nielsen OV
Schaffalitzky de Muckadell OB
Source :
The Journal of physiology [J Physiol] 1981 Sep; Vol. 318, pp. 327-37.
Publication Year :
1981

Abstract

1. A method for the isolation and vascular perfusion of the porcine pancreas and duodenum was developed. 2. The oxygen consumption of the whole preparation was similar to that of the pancreas alone, and since the duodenal arteriovenous oxygen deficit was similar to that of the total preparation, it was concluded that the duodenum respired adequately. 3. The duodenum rapidly absorbed luminally administered radioactive glucose, and this absorption was strongly inhibited by ouabain and phloridzin. 4. The duodenum secreted secretin rapidly in response to hydrochloric acid, but did not respond to any other luminal stimuli, including lipids, proteins, carbohydrates and bile. Neither was secretin release stimulated by intra-arterially injected acetylcholine. 5. By gel permeation chromatography the release immunoreactive secretin behaved identically to pure natural secretin, indicating that the tissue form and the circulating form have identical molecular size. 6. It is concluded that this model offers an unique opportunity to study the endocrine secretion of the duodenum.

Details

Language :
English
ISSN :
0022-3751
Volume :
318
Database :
MEDLINE
Journal :
The Journal of physiology
Publication Type :
Academic Journal
Accession number :
7320893
Full Text :
https://doi.org/10.1113/jphysiol.1981.sp013867