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Simultaneous determination of chloroquine and its desethyl metabolite in human plasma by gas chromatography.
Simultaneous determination of chloroquine and its desethyl metabolite in human plasma by gas chromatography.
- Source :
-
Journal of chromatography [J Chromatogr] 1981 Nov 13; Vol. 226 (1), pp. 91-7. - Publication Year :
- 1981
-
Abstract
- A gas chromatographic method for the simultaneous determination of chloroquine and its metabolite desethylchloroquine in plasma described. Chloroquine, desethylchloroquine and internal standard are extracted as bases with n-hexane-pentanol (90:10) and then back-extracted to an acid aqueous phase. The aqueous phase is made alkaline and after re-extraction into chloroform and evaporation of the chloroform, acylation with trifluoroacetic anhydride is performed. Separation is achieved on an OV-17 column at 250 degrees C. Chloroquine and desethylchloroquine can be determined down to 0.1-0.2 mumol/l (30-60 ng/ml), with a coefficient of variation of 12%, using a nitrogen detector. The method shows good correlation (r = 0.98) with a recently developed liquid chromatographic method.
Details
- Language :
- English
- Volume :
- 226
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of chromatography
- Publication Type :
- Academic Journal
- Accession number :
- 7320158
- Full Text :
- https://doi.org/10.1016/s0378-4347(00)84209-7