Back to Search
Start Over
Studies on the structures of the carbohydrate moiety of human prothrombin.
- Source :
-
Journal of biochemistry [J Biochem] 1981 Oct; Vol. 90 (4), pp. 1023-31. - Publication Year :
- 1981
-
Abstract
- Human prothrombin contains three asparagine-linked sugar chains in one molecule. The sugar chains were quantitatively liberated as radioactive oligosaccharides from the polypeptide moiety by hydrazinolysis followed by N-acetylation and Nab3H4 reduction. All of the oligosaccharides contain N-acetylneuraminic acid. The neutral oligosaccharides obtained from all acidic oligosaccharides by sialidase digestion are identical. By the combination of sequential exoglycosidase digestion and methylation analysis, the structures of the asparagine-linked sugar chains of human prothrombin were confirmed to be as follows: (sequence in text).
Details
- Language :
- English
- ISSN :
- 0021-924X
- Volume :
- 90
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 7309709
- Full Text :
- https://doi.org/10.1093/oxfordjournals.jbchem.a133554