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D-glucose uptake in human liver cell cultures.
- Source :
-
In vitro [In Vitro] 1981 Sep; Vol. 17 (9), pp. 745-51. - Publication Year :
- 1981
-
Abstract
- The kinetics parameters for D-glucose uptake were studied in human liver cell cultures under strictly defined experimental conditions. Using a wide concentration range (0.005 to 30 mmol/l), the kinetic data obtained suggested strongly that D-glucose in human liver cell cultures can be transported by two separate systems. For the high-affinity system, the apparent Km was 0.645 +/- 0.21 mmol/l and the Vmax, 12.49 +/- 3.74 nmol/mg protein per min. For the low-affinity system, the apparent Km was 6.91 +/- 0.58 mmol/l and the Vmax, 79.90 +/- 5.27 nmol/mg protein per min. At a concentration of 2.1 x 10(-7) mol/l, cytochalasin B preferentially inhibited the high-affinity D-glucose site or transport system. The time course of D-glucose uptake, studied in two cell lines from patients with hereditary fructose intolerance, was significantly higher than for the control lines.
Details
- Language :
- English
- ISSN :
- 0073-5655
- Volume :
- 17
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- In vitro
- Publication Type :
- Academic Journal
- Accession number :
- 7298055
- Full Text :
- https://doi.org/10.1007/BF02618440