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Detection of complementary RNA intermediates of viroid replication by Northern blot hybridization.
- Source :
-
Bioscience reports [Biosci Rep] 1981 Apr; Vol. 1 (4), pp. 327-36. - Publication Year :
- 1981
-
Abstract
- Molecular hybridization by the Northern blot technique in combination with 125I-labeled PSTV (+) RNA and 32P-labeled PSTV cDNA as probes has been applied to detect viroid-specific sequences in healthy and viroid(PSTV)-infected tomato plants. Conditions are described which allow differentiation of (+) and (-) viroid sequences on the basis of the different thermostabilities of the corresponding hybrid molecules. By this experimental approach, it is documented that no viroid-specific DNA sequences can be detected and that viroid replication proceeds via complementary RNA intermediates. Out of the seven (-) RNA species found, six are apparently larger than the circular viroid (+) RNA and one is about the same size as the linear (+) RNA molecule.
Details
- Language :
- English
- ISSN :
- 0144-8463
- Volume :
- 1
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Bioscience reports
- Publication Type :
- Academic Journal
- Accession number :
- 7295896
- Full Text :
- https://doi.org/10.1007/BF01114872