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Detection of complementary RNA intermediates of viroid replication by Northern blot hybridization.

Authors :
Rohde W
Sänger HL
Source :
Bioscience reports [Biosci Rep] 1981 Apr; Vol. 1 (4), pp. 327-36.
Publication Year :
1981

Abstract

Molecular hybridization by the Northern blot technique in combination with 125I-labeled PSTV (+) RNA and 32P-labeled PSTV cDNA as probes has been applied to detect viroid-specific sequences in healthy and viroid(PSTV)-infected tomato plants. Conditions are described which allow differentiation of (+) and (-) viroid sequences on the basis of the different thermostabilities of the corresponding hybrid molecules. By this experimental approach, it is documented that no viroid-specific DNA sequences can be detected and that viroid replication proceeds via complementary RNA intermediates. Out of the seven (-) RNA species found, six are apparently larger than the circular viroid (+) RNA and one is about the same size as the linear (+) RNA molecule.

Details

Language :
English
ISSN :
0144-8463
Volume :
1
Issue :
4
Database :
MEDLINE
Journal :
Bioscience reports
Publication Type :
Academic Journal
Accession number :
7295896
Full Text :
https://doi.org/10.1007/BF01114872