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Comparative action of reducing agents on fibrillar human bronchial mucus under dissociating and non-dissociating conditions.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1981 May 29; Vol. 668 (3), pp. 413-9. - Publication Year :
- 1981
-
Abstract
- Gel=like or fibrillar human bronchial mucus was reduced with mercaptoethanol under dissociating and non-dissociating conditions. Bronchial mucus was solubilized in both conditions; but reduction in phosphate buffer induced more extensive depolymerization of mucus glycoproteins than obtained under reduction in guanidine. Moreover, mucins prepared by reduction in non-dissociating conditions contained less amino acid and more carbohydrate. These data strongly suggest that reduction under non-dissociating conditions does not act only on disulfide bridges but also induces the activation of a mucolytic enzymatic system. They also would explain some of the discrepancies observed in the molecular weight determination and chemical composition of human bronchial mucus glycoproteins purified after reduction of gel-like mucus under different conditions.
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 668
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 7236718
- Full Text :
- https://doi.org/10.1016/0005-2795(81)90175-6