Back to Search
Start Over
Determination of high density lipoprotein-cholesterol in human plasma stored at -70 degrees C.
- Source :
-
Journal of lipid research [J Lipid Res] 1982 Nov; Vol. 23 (8), pp. 1236-42. - Publication Year :
- 1982
-
Abstract
- We determined the effect of storage at -70 degrees C on the determination of plasma high density lipoprotein (HDL)-cholesterol. Plasma from 106 subjects was stored for 1, 6, and 12 months, then treated with heparin and MnCl2 to remove other lipoproteins, and HDL-cholesterol was measured. The mean HDL-cholesterol level decreased by 2.9% after 1 month, and by 5.1% after 12 months. The magnitude and direction of the changes were not constant, but were correlated primarily with HDL-cholesterol concentration. After 1 month, samples with HDL-cholesterol levels below 40 mg/dl tended to increase, and those above this value tended to decrease. By 12 months, only those samples with HDL-cholesterol below 22 mg/dl tended to increase. Linear regression analysis indicated changes of 0.9-1.5 mg/dl for each 10 mg/dl initial HDL-cholesterol concentration. Storage of heparin-MnCl2 supernatants, rather than unfractionated plasma, minimized these changes. The mean HDL-cholesterol of stored heparin-MnCl2 supernatants was 3.3% lower after 12 months, and the change was constant regardless of lipoprotein concentration. The findings suggest the possible occurrence of changes in the heparin-MnCl2 precipitability of lipoproteins during storage, which produce errors in HDL-cholesterol analyses, and indicate that samples can be stored more satisfactorily if the other lipoproteins are removed first.
Details
- Language :
- English
- ISSN :
- 0022-2275
- Volume :
- 23
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of lipid research
- Publication Type :
- Academic Journal
- Accession number :
- 7175380