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The reconstitution of histone H3-H4 tetramer from acid extracted histones in the absence of urea.
The reconstitution of histone H3-H4 tetramer from acid extracted histones in the absence of urea.
- Source :
-
FEBS letters [FEBS Lett] 1982 Aug 16; Vol. 145 (1), pp. 131-6. - Publication Year :
- 1982
-
Abstract
- Simple mixing of acid purified histones H3 and H4 in equimolar quantities at low ionic strength near pH 7 does not yield the tetramer but rather a high Mr aggregate. Dialysis of acid extracted total or core histones into 2 M NaCl 150 mM phosphate (pH 7.4) followed by fractionation of the histone complexes at lower ionic strength (150 mM NaCl) results in an H3-H4 tetramer of a structure identical to that derived from salt-extracted histones. Dialysis of acid extracted total or core histones directly into the lower ionic strength buffer with subsequent fractionation, results in H3-H4 tetramer of closely similar structure.
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 145
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 7128818
- Full Text :
- https://doi.org/10.1016/0014-5793(82)81221-0