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The reconstitution of histone H3-H4 tetramer from acid extracted histones in the absence of urea.

The reconstitution of histone H3-H4 tetramer from acid extracted histones in the absence of urea.

Authors :
Lindsey GG
Thompson P
Purves LR
von Holt C
Source :
FEBS letters [FEBS Lett] 1982 Aug 16; Vol. 145 (1), pp. 131-6.
Publication Year :
1982

Abstract

Simple mixing of acid purified histones H3 and H4 in equimolar quantities at low ionic strength near pH 7 does not yield the tetramer but rather a high Mr aggregate. Dialysis of acid extracted total or core histones into 2 M NaCl 150 mM phosphate (pH 7.4) followed by fractionation of the histone complexes at lower ionic strength (150 mM NaCl) results in an H3-H4 tetramer of a structure identical to that derived from salt-extracted histones. Dialysis of acid extracted total or core histones directly into the lower ionic strength buffer with subsequent fractionation, results in H3-H4 tetramer of closely similar structure.

Details

Language :
English
ISSN :
0014-5793
Volume :
145
Issue :
1
Database :
MEDLINE
Journal :
FEBS letters
Publication Type :
Academic Journal
Accession number :
7128818
Full Text :
https://doi.org/10.1016/0014-5793(82)81221-0