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The demonstration of protein-bound 99Mo-di- and trithiomolybdate in sheep plasma after the infusion of 99M0-labelled molybdate into the rumen.
- Source :
-
The British journal of nutrition [Br J Nutr] 1982 Sep; Vol. 48 (2), pp. 391-7. - Publication Year :
- 1982
-
Abstract
- 1. Protein-bound, trichloracetic acid- (TCA) insoluble 99Mo appeared in plasma a few hours after the introduction of 99Mo-labelled molybdate (30 mg Mo) into the rumen of sheep maintained on a basic diet supplemented with elemental sulphur (3 g S/d). 2. Most of the 99Mo could be displaced from its protein carrier in vitro and the labelled compounds displaced were identified by sephadex G-25 chromatography as di- and trithiomolybdate. Tetrathiomolybdate was not detected. 3. In control experiments protein-bound, TCA-insoluble 99Mo predominated in plasma after the direct administration of [99Mo]tetrathiomolybdate, either into the rumen or intravenously. The 99Mo could be displaced in vitro and [99Mo]tetrathiomolybdate identified, although [99Mo]trithiomolybdate was also present. The study provides direct evidence of thiomolybdate synthesis and absorption in ruminants in vivo.
Details
- Language :
- English
- ISSN :
- 0007-1145
- Volume :
- 48
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The British journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 7115663
- Full Text :
- https://doi.org/10.1079/bjn19820122