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[Enzyme-immunoassay of grass-pollen and mites antibodies in rabbit antisera on magnetic polyacrylamide agarose beads (author's transl)].

Authors :
Guesdon JL
David B
Lapeyre J
Source :
Annales d'immunologie [Ann Immunol (Paris)] 1977 Jul-Oct; Vol. 128C (4-5), pp. 799-810.
Publication Year :
1977

Abstract

Complex antigens were coupled to polyacrylamide agarose beads which had been made magnetic with iron oxide. Those beads were then used in an enzyme-immunoassay for quantitation of specific antibodies to grass-pollen and mites in rabbit antisera. The results obtained by a direct or an inhibition technique proved the specificity of this assay towards homologous antigens. Moreover, cross-reactions between the various species of grass-pollen could be shown. One advantage of this simple method is that it may avoid use of radioactive compounds.

Details

Language :
French
ISSN :
0300-4910
Volume :
128C
Issue :
4-5
Database :
MEDLINE
Journal :
Annales d'immunologie
Publication Type :
Academic Journal
Accession number :
71011