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Induction of autoantibody-producing cells after the coculture of haptenated and normal human mononuclear leukocytes.
- Source :
-
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) [Proc Soc Exp Biol Med] 1981 Oct; Vol. 168 (1), pp. 131-6. - Publication Year :
- 1981
-
Abstract
- The coculture of normal human peripheral blood mononuclear leukocytes (PBL) and autologous mononuclear leukocytes coupled to the trinitrophenyl (TNP) hapten (TNP-PBL) was found to induce a polyclonal activation of antibody-producing cells. The polyclonal activation of antibody-producing cells was demonstrated by detecting the induction of cells producing antibody to sheep red blood cells using a complement-dependent, direct, hemolytic plaque-forming cell (PFC) assay. A ratio of four normal to one haptenated mononuclear leukocyte was found to be optimal for inducing the polyclonal activation of antibody-producing cell in these cultures. The plaque-forming cells assay in these experiments utilized monolayers of indicator red cells. Further evidence for the polyclonal induction of antibody-producing cells by TNP-PBL was provided by demonstrating PFC on monolayers of not only sheep red blood cells, but also autologous human red cells, bromelain-treated autologous red cells, TNP-coupled human and sheep red cells, and human autologous red cells coupled to human heat-aggregated IgG with chromic chloride. Thus cells secreting antibody to TNP, human red cells, and human IgG were induced. Anti-IgG and anti-human red cell-producing cells were first detected on Day 2 of culture and were still present on Day 9. Mononuclear leukocytes altered by chemical haptenation polyclonally stimulate normal mononuclear leukocytes to become antibody-producing cells. This polyclonal stimulation of antibody-producing cells includes cells producing antibodies to human IgG and human autologous red blood cells suggesting that autoantibody-producing cells are induced.
- Subjects :
- Animals
Bromelains pharmacology
Cells, Cultured
Erythrocytes immunology
Hemolytic Plaque Technique
Humans
Immunoglobulin G immunology
Nitrophenols immunology
Rheumatoid Factor immunology
Sheep
Antibody-Producing Cells immunology
Autoantibodies biosynthesis
Haptens immunology
Leukocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0037-9727
- Volume :
- 168
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 7033965
- Full Text :
- https://doi.org/10.3181/00379727-168-41247