Mapping the structural genes coding for the major urinary proteins in the mouse: combined use of recombinant inbred strains and somatic cell hybrids.

Authors :: Bennett KL
Lalley PA
Barth RK
Hastie ND
Source :: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1982 Feb; Vol. 79 (4), pp. 1220-4.
Publication Year :: 1982
Abstract: We have mapped the multiple (15-25) genes coding for the hormonally regulated major urinary proteins (MUPs) of the mouse by using a cloned cDNA probe. By Southern blot analysis of DNA from Chinese hamster-mouse somatic cell hybrids, all of the MUP genes were found to be on chromosome 4. Different inbred mouse strains showed DNA polymorphism in their MUP Southern hybridization pattern. Analysis of recombinant inbred strains derived from these parent strains has shown that all the polymorphisms are linked to the MUP-a locus on chromosome 4. The combination of these mapping techniques should be applicable to many cloned DNA sequences.

Subjects :: Animals
Chromosome Mapping
DNA
Genetic Linkage
Mice
Mice, Inbred AKR
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Inbred Strains
Nucleic Acid Hybridization
Polymorphism, Genetic
Proteins metabolism
Proteinuria
Recombination, Genetic
Genes
Proteins genetics

Language :: English
ISSN :: 0027-8424
Volume :: 79
Issue :: 4
Database :: MEDLINE
Journal :: Proceedings of the National Academy of Sciences of the United States of America
Publication Type :: Academic Journal
Accession number :: 6951169
Full Text :: https://doi.org/10.1073/pnas.79.4.1220

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