The effect of indomethacin on the biosynthesis and metabolism of prostaglandin F2alpha in man.

Healthy volunteers received 60 microgram of [8,10,10(-2)H3] PGF2alpha by intravenous infusion both before and during a course of treatment with indomethacin (200 mg/day). Excretion of deuterated 5alpha, 7alpha-dihydroxy-11-ketotetranor-prostane-1, 16-dioic acid in urine was quantified by GC-MS using a reverse stable isotope dilution procedure. Indomethacin was found to have no detectable effect on the metabolism of the labelled PGF2alpha whereas output of the endogenous metabolite was markedly reduced by the effect of the drug on prostaglandin biosynthesis.