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Adenovirus DNA replication in vitro: characterization of a protein covalently linked to nascent DNA strands.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1980 Sep; Vol. 77 (9), pp. 5105-9. - Publication Year :
- 1980
-
Abstract
- The 5' terminus of each nascent daughter strand of adenovirus DNA replicated in vitro is covalently linked to a protein with an apparent Mr of 80,000. This protein may represent a precursor to the 55,000-dalton protein known to be linked to the 5' ends of mature adenovirus DNA strands. Partial proteolysis experiments indicate that the 80,000-dalton and 55,000-dalton proteins are structurally related. Furthermore, both proteins are attached to DNA by the same linkage: a phosphodiester bond between the beta-OH of a serine residue in the protein and the 5'-OH of the terminal deoxycytidine residue of the DNA. The role of the 80,000-dalton protein in adenovirus DNA replication is not yet clear, although one reasonable possibility is that it serves as the primer for daughter strand synthesis.
- Subjects :
- Electrophoresis, Polyacrylamide Gel
Models, Biological
Molecular Weight
Protein Precursors isolation & purification
Adenoviruses, Human metabolism
DNA, Viral biosynthesis
Deoxyribonucleoproteins isolation & purification
Nucleoproteins isolation & purification
Viral Proteins isolation & purification
Virus Replication
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 77
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 6933548
- Full Text :
- https://doi.org/10.1073/pnas.77.9.5105