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Intracellular pH and free calcium changes in single cells using quene 1 and quin 2 probes and fluorescence microscopy.
- Source :
-
FEBS letters [FEBS Lett] 1983 Sep 05; Vol. 161 (1), pp. 21-7. - Publication Year :
- 1983
-
Abstract
- Photometric fluorescence microscopy has been used to measure intracellular pH (pHi) and free calcium concentrations [( Ca]i) in individual mouse thymocytes and 2H3 rat basophil leukaemic cells containing indicators for pH (quene 1) or calcium (quin 2). The pHi and [Ca]i measurements in individual 2H3 cells and mouse thymocytes and their responses to various stimuli were consistent with the corresponding data obtained from suspensions of these cells measured in a spectrofluorimeter. Photometric fluorescence microscopy of these indicators in individual cells provides a sensitive and fast method of following pHi and [Ca]i responses in individual cells.
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 161
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 6884525
- Full Text :
- https://doi.org/10.1016/0014-5793(83)80722-4