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The incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and alpha.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1983 Jun 25; Vol. 11 (12), pp. 4185-93. - Publication Year :
- 1983
-
Abstract
- The modified nucleoside 5'-triphosphates O6-MedGTP ad O4-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and alpha in an in vitro assay. O6-MedGMP is only incorporated into newly-synthesized DNA-like material in the presence of templates containing thymine bases. Similarly O4-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of O6-MeG ad O4-MeT.
- Subjects :
- DNA Replication drug effects
Deoxyguanine Nucleotides pharmacology
Thymine Nucleotides pharmacology
DNA Polymerase I metabolism
DNA Polymerase II metabolism
DNA-Directed DNA Polymerase metabolism
Deoxyguanine Nucleotides metabolism
Thymidine Monophosphate metabolism
Thymine Nucleotides metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 11
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 6866769
- Full Text :
- https://doi.org/10.1093/nar/11.12.4185