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Localization of human variable and constant region immunoglobulin heavy chain genes on subtelomeric band q32 of chromosome 14.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1982 Dec 20; Vol. 10 (24), pp. 8155-70. - Publication Year :
- 1982
-
Abstract
- Analysis of a group of human/rodent somatic cell hybrids with nucleic acid probes prepared from cloned human variable region (VH), junctional (JH), and constant region (C epsilon) heavy chain immunoglobulin genes indicates that all of these IgH genes are localized on the subtelomeric (q32) band of chromosome 14. Somatic cell hybrids were isolated in selective medium after fusing human fibroblasts with hprt- Chinese hamster cells. The human parental cells contained two translocation chromosomes representing a reciprocal translocation between chromosomes X and 14. Only those hybrid cell lines retaining a complete human autosome 14 or the X/14 translocation chromosome (i.e. containing band 14q32) retained the human IgH genes. Retention of these genes did not correlate with the presence of the other translocation chromosome, 14/X. These results indicate that all human IgH genes (VH, JH, and CH) map to the same chromosomal band (14q32) which is commonly involved in reciprocal translocations with human chromosome 8 (8q24) in B-cell neoplasms.
- Subjects :
- Animals
Cell Line
Cricetinae
Cricetulus
Fibroblasts metabolism
Humans
Hybrid Cells
Karyotyping
Translocation, Genetic
Binding Sites, Antibody genetics
Chromosomes, Human, 13-15
Genes
Immunoglobulin Constant Regions genetics
Immunoglobulin Heavy Chains genetics
Immunoglobulin Variable Region genetics
Immunoglobulins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 10
- Issue :
- 24
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 6819544
- Full Text :
- https://doi.org/10.1093/nar/10.24.8155