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Characterization and genetic mapping of fructose phosphotransferase mutations in Pseudomonas aeruginosa.
- Source :
-
Journal of bacteriology [J Bacteriol] 1982 Mar; Vol. 149 (3), pp. 897-905. - Publication Year :
- 1982
-
Abstract
- Pseudomonas aeruginosa transports and phosphorylates fructose via a phosphoenolpyruvate-dependent fructose phosphotransferase system (PTS). Mutant strains deficient in both PTS activity and glucose-6-phosphate dehydrogenase activity were isolated and were used to select mannitol-utilizing revertant strains singly deficient in PTS activity. These mutants were unable to utilize fructose as a carbon source and failed to accumulate exogenously provided [14C]fructose, and crude cell extracts lacked phosphoenolpyruvate-dependent fructose PTS activity. Thus, the PTS was essential for the uptake and utilization of exogenously provided fructose by P. aeruginosa. Mutations at a locus designated pts, which resulted in a loss of PTS activity, exhibited 57% linkage to argF at 55 min on the chromosome in plasmid R68.45-mediated conjugational crosses. The pts mutations in four independently isolated mutant strains exhibited from 11 to 20% linkage to argF, and one of these mutations exhibited 3% linkage to lys-9015 in phage F116L-mediated transductional crosses.
- Subjects :
- Chromosome Mapping
Conjugation, Genetic
Fructose metabolism
Genetic Linkage
Mutation
Phosphoenolpyruvate Sugar Phosphotransferase System metabolism
Pseudomonas aeruginosa genetics
Pseudomonas aeruginosa growth & development
Transduction, Genetic
Genes, Bacterial
Phosphoenolpyruvate Sugar Phosphotransferase System genetics
Pseudomonas aeruginosa enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 149
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 6801014
- Full Text :
- https://doi.org/10.1128/jb.149.3.897-905.1982