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Demonstration and partial characterization of a 7,12-dimethylbenz[alpha]anthracene-binding protein in rat adrenal.
- Source :
-
Chemico-biological interactions [Chem Biol Interact] 1981 Oct; Vol. 37 (1-2), pp. 55-65. - Publication Year :
- 1981
-
Abstract
- Rat adrenal cytosol was found to contain a 7,12-dimethylbenz[a]-anthracene (DMBA)-binding protein which is characterized by a pI of 7.2, a Kd-value of 3 microM and a maximal capacity of about 47 pmol/mg protein. The binding is highly specific for DMBA and is not displaced by 3-methylcholanthrene (MC), benz[a]pyrene (BP) or other polycyclic hydrocarbons. Likewise, various androgens, estrogens or glucocorticoids have no effect on the DMBA binding. It is proposed that the DMBA-binding protein may have a role in the toxic effects of DMBA or DMBA metabolites in adrenal and possibly in other DMBA-sensitive organs as well.
- Subjects :
- 9,10-Dimethyl-1,2-benzanthracene isolation & purification
9,10-Dimethyl-1,2-benzanthracene metabolism
Adrenal Glands drug effects
Animals
Carrier Proteins metabolism
Chromatography, Gel
Cytosol metabolism
Hot Temperature
Isoelectric Focusing
Male
Polycyclic Compounds pharmacology
Rats
Rats, Inbred Strains
Steroids pharmacology
Trypsin metabolism
Adrenal Glands metabolism
Carrier Proteins isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0009-2797
- Volume :
- 37
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Chemico-biological interactions
- Publication Type :
- Academic Journal
- Accession number :
- 6793253
- Full Text :
- https://doi.org/10.1016/0009-2797(81)90165-4