Back to Search
Start Over
Cardiolipin, a major phospholipid of Gram-positive bacteria that is not readily extractable.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1980 Nov 07; Vol. 620 (2), pp. 332-7. - Publication Year :
- 1980
-
Abstract
- Extraction of phospholipids from stationary phase grown cells of the Gram+ bacteria, Bacillus megaterium, Bacillus subtilis, Bacillus cereus and Micrococcus lysodeikticus was found to be incomplete with various commonly used extraction procedures. Phosphatidylglycerol and phosphatidylethanolamine were readily extracted but up to 95% of the cardiolipin appeared to be retained within the cell residue. Extraction of the cardiolipin could be slightly enhanced by increasing the temperature or the acidity of the extraction solutions but complete extraction was obtained only after lysozyme treatment of intact cells or cell residues remaining after extraction. In addition complete extraction could be observed in the case of cells harvested in the early logarithmic phase. Freeze-fracture electron microscopy was carried out on the cell residue remaining after extraction of all phospholipids except cardiolipin. A fracture plane through the plasma membrane could not be observed anymore. Instead fracture planes through lipid vesicles were observed. These vesicles reside within the remnants of the cytoplasm and consist most likely of the non-extracted cardiolipin.
- Subjects :
- Bacillus cereus analysis
Bacillus megaterium analysis
Bacillus megaterium ultrastructure
Bacillus subtilis analysis
Freeze Fracturing
Microscopy, Electron
Phospholipids isolation & purification
Species Specificity
Bacillus analysis
Cardiolipins isolation & purification
Micrococcus analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 620
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 6776994
- Full Text :
- https://doi.org/10.1016/0005-2760(80)90215-5