Identification of prosomatostatin in pancreatic islets.

Authors :: Patzelt C
Tager HS
Carroll RJ
Steiner DF
Source :: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1980 May; Vol. 77 (5), pp. 2410-4.
Publication Year :: 1980
Abstract: A 12.5-kilodalton protein not related to insulin or glucagon was detected in pulse-chase-labeled rat islets of Langerhans. Although this protein reacted poorly with various somatostatin antisera, analysis of two-dimensional peptide maps showed that it contains all of the tryptic fragments of somatostatin, which is located at its COOH terminus. Proteolytic conversion of the putative prosomatostatin, which took place parallel to the processing of proinsulin and proglucagon in pulse-chase experiments, coincided with the appearance of newly synthesized somatostatin and proceeded without the apparent involvement of major intermediate forms.

Subjects :: Amino Acid Sequence
Islets of Langerhans cytology
Molecular Weight
Peptide Fragments analysis
Growth Hormone-Releasing Hormone metabolism
Islets of Langerhans metabolism
Protein Precursors metabolism

Language :: English
ISSN :: 0027-8424
Volume :: 77
Issue :: 5
Database :: MEDLINE
Journal :: Proceedings of the National Academy of Sciences of the United States of America
Publication Type :: Academic Journal
Accession number :: 6771754
Full Text :: https://doi.org/10.1073/pnas.77.5.2410