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HMGCoA reductase and cholesterol 7 alpha-hydroxylase in human liver.
- Source :
-
Life sciences [Life Sci] 1984 May 21; Vol. 34 (21), pp. 2075-81. - Publication Year :
- 1984
-
Abstract
- The activities of HMGCoA reductase and cholesterol 7 alpha-hydroxylase were assayed in liver biopsies of patients with or without cholestyramine treatment. The active dephosphorylated form of HMGCoA reductase and the activity of cholesterol 7 alpha-hydroxylase were under the detection limits in untreated subjects. After cholestyramine treatment activities of both enzymes were stimulated and the active form of HMGCoA reductase became detectable in four out of the five tested patients. In two subjects who received cholestyramine, the effect of exogenously added [4-14C] cholesterol on cholesterol 7 alpha-hydroxylase was tested. In the presence of Tween 80, the detergent by which [14C]cholesterol was suspended, the enzyme activity was profoundly inhibited and synthesis of 7 alpha-hydroxycholesterol was extremely low.
- Subjects :
- Adult
Aged
Cholelithiasis enzymology
Cholesterol blood
Female
Humans
Liver drug effects
Male
Microsomes, Liver enzymology
Middle Aged
Stomach Ulcer enzymology
Triglycerides blood
Cholesterol 7-alpha-Hydroxylase metabolism
Cholestyramine Resin
Hydroxymethylglutaryl CoA Reductases metabolism
Liver enzymology
Steroid Hydroxylases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0024-3205
- Volume :
- 34
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 6727552
- Full Text :
- https://doi.org/10.1016/0024-3205(84)90372-2