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Poly(dA-dT) has a right-handed B conformation in solution: a two-dimensional NMR study.
- Source :
-
Biochemistry [Biochemistry] 1984 Feb 28; Vol. 23 (5), pp. 791-6. - Publication Year :
- 1984
-
Abstract
- The structure of poly(dA-dT) molecules in solution has been probed by using two-dimensional nuclear Overhauser effect spectroscopy. Cross-relaxation patterns arising from internucleotide and intranucleotide interactions are used to assign the sugar proton resonances and to deduce various structural features. The numerous proton-proton interactions that are observed indicate that poly(dA-dT) is a right-handed helix with a B-type conformation. Both the adenine and thymine nucleotides are in an anti conformation. Although slight differences in the purine-sugar and pyrimidine-sugar intranucleotide interactions are observed, the large differences in the sugar pucker of the adenine vs. thymine nucleotide suggested by some models [Klug, A., Jack, A., Viswamitra, M. A., Kennard, O., Shakked, Z., & Steitz, T. A. (1979) J. Mol. Biol. 131, 669-680] are not evident in the solution structure of poly(dA-dT). In the low-temperature spectra there is unexpected evidence for cross-strand AH2-AH2 interactions.
Details
- Language :
- English
- ISSN :
- 0006-2960
- Volume :
- 23
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6712923
- Full Text :
- https://doi.org/10.1021/bi00300a001