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Synthesis of a metallothionein-like protein by developing turkey embryos maintained in long-term, shell-less culture.
- Source :
-
Journal of pediatric gastroenterology and nutrition [J Pediatr Gastroenterol Nutr] 1984; Vol. 3 (1), pp. 128-36. - Publication Year :
- 1984
-
Abstract
- Turkey embryos were maintained in long-term, shell-less culture to the age of 17 days. At this time, 10 cultures were given saline (controls), and 10 cultures were given 30 micrograms zinc in saline (+Zn). In addition, all cultures received 5 microCi each of [35S]cystine and 65Zn dissolved in the saline and applied to the chorioallantoic membrane. After a further 20 h of incubation, no significant differences were found for embryo, liver, or yolk sac weights nor for relative liver size, hematocrit, serum protein, or serum concentrations of zinc and copper for the control and + Zn cultures. The addition of zinc to the cultures significantly (p less than 0.01) elevated hepatic and yolk sac cytosol 65Zn and zinc concentrations. More than 70% of the increased 65Zn and zinc in the cytosol of liver and yolk sac could be accounted for as that bound to a low-molecular-weight metallothionein (MT)-like protein. Added zinc significantly (p less than 0.01) increased the incorporation of [35S]cystine and 65Zn into the MT-like protein from liver and yolk sac indicating de novo synthesis of this protein. Liver and yolk sac MT-like proteins eluted from DEAE Sephadex A-25 columns as a single predominant species. The synthesis of an MT-like protein by liver and yolk sac suggests a role in embryonic zinc homeostasis. Moreover, shell-less culture of the avian embryo appears to be a useful method for studying zinc metabolism during periods of embryonic growth and development.
Details
- Language :
- English
- ISSN :
- 0277-2116
- Volume :
- 3
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of pediatric gastroenterology and nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 6694041
- Full Text :
- https://doi.org/10.1097/00005176-198401000-00025