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Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice.
- Source :
-
Cellular immunology [Cell Immunol] 1984 Mar; Vol. 84 (1), pp. 32-40. - Publication Year :
- 1984
-
Abstract
- The relationship between colony formation (cloning) of B cells and their activation in murine autoimmunity was investigated in MRL-lpr/lpr and MRL.xid mice. Cells from MRL-lpr/lpr mice showed similar requirements for in vitro growth as normal CBA/J and BALB/c cells, with maximal colony formation in the presence of the supporting factors lipopolysaccharide and sheep red blood cells. The frequency of colony-forming cells from MRL-lpr/lpr spleens or hapten-specific B-cell preparations was slightly greater than the two normal control strains, with this difference significant only for a comparison of BALB/c and MRL-lpr/lpr spleens. In contrast, MRL-lpr/lpr mice bearing the xid gene for B-cell immunodeficiency (MRL.xid) had markedly reduced B-cell colony formation. These mice nevertheless expressed anti-DNA antibodies, although at levels reduced from that of MRL-lpr/lpr controls. These results indicate that enhanced in vitro colony formation need not accompany B-cell hyperactivity in murine autoimmune disease and that autoantibody production can occur in mice with impairment in this growth property.
- Subjects :
- Animals
Autoimmune Diseases genetics
B-Lymphocytes cytology
Clone Cells cytology
Clone Cells immunology
Colony-Forming Units Assay
Female
Immunologic Deficiency Syndromes immunology
Male
Mice
Mice, Inbred BALB C
Mice, Inbred CBA
Spleen cytology
Autoimmune Diseases immunology
B-Lymphocytes immunology
Immunologic Deficiency Syndromes genetics
Lymphocyte Activation
Mice, Mutant Strains immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-8749
- Volume :
- 84
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 6607779
- Full Text :
- https://doi.org/10.1016/0008-8749(84)90074-1