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Generation of alloreactive cytotoxic T lymphocytes: evidence for a differentiation factor distinct from IL 2.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1983 Feb; Vol. 130 (2), pp. 763-7. - Publication Year :
- 1983
-
Abstract
- In this report we provide evidence that the generation of cytotoxic T lymphocytes (CTL) involves a differentiation signal in addition to a proliferative signal, and that the differentiation signal is mediated by a factor distinct from interleukin 2 (IL 2). Generation of CTL from nylon wool-nonadherent splenocytes stimulated by heat-treated thymocytes did not correlate with the addition of IL 2-containing supernatants to this factor-dependent bioassay. IL 2 from two different sources and prepared by different methods (purified IL 2 from the LBRM33 cell line and partially purified IL 2 from the EL4 cell line) was not sufficient to generate a cytotoxic response in the bioassay. The inability of IL 2 to generate a cytotoxic response was not due to the use of heat-treated stimulator cells because similar results were obtained with UV-treated cells. As we previously reported, supernatant fluids containing a differentiation/activation factor did generate CTL from nylon wool-nonadherent splenocytes. Supernatant fluid from co-cultures of activated T cells and M phi as well as from cultures of activated M phi contained this CTL helper factor; however, only the former supernatant contained IL 2 activity. These results suggest a factor distinct from IL 2 is providing a differentiation/activation signal that is necessary for CTL development. Although IL 2 was not effective at generating CTL from nylon wool-nonadherent splenocytes, it was capable in some, but not all, experiments of generating a cytotoxic response from unseparated splenocytes. The ability of IL 2 to induce a cytotoxic response from unseparated splenocytes appeared to correlate with the presence of a weak background response seen when unseparated cells were used as responder cells.
- Subjects :
- Animals
Cell Adhesion
Cell Differentiation
Cell Separation
Interleukin-1 analysis
Interleukin-2 analysis
Macrophage Activation
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Inbred DBA
Spleen cytology
Interleukin-1 pharmacology
Interleukin-2 pharmacology
Lymphocyte Activation
T-Lymphocytes, Cytotoxic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 130
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 6600253