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Determination of free and total cholesterol in human bile samples using an enzyme electrode.
- Source :
-
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 1983 Jul 31; Vol. 132 (1), pp. 7-15. - Publication Year :
- 1983
-
Abstract
- A reliable cholesterol electrode was assembled with a Clark type oxygen electrode and cholesterol oxidase immobilised on a nylon net fixed on the electrode surface. Determination of free (unesterified) and total (esterified and unesterified) cholesterol in human bile samples was performed. Results were compared with the enzymatic-spectrophotometric procedure based on the Roeschlau method. The electrode has a usable life of more than two weeks, and more than 300 determinations could be performed. The CV values for free and total cholesterol in bile samples is 5-6%. The comparison between the proposed procedure and the spectrophotometric procedure is fair for the half of the results (delta less than 5%) and acceptable in the other half (delta less than 25%).
Details
- Language :
- English
- ISSN :
- 0009-8981
- Volume :
- 132
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Clinica chimica acta; international journal of clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6577990
- Full Text :
- https://doi.org/10.1016/0009-8981(83)90227-9