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On the mechanism of tRNATrp aminoacylation catalysed by beef tryptophanyl-tRNA synthetase using presteady-state kinetics.
- Source :
-
FEBS letters [FEBS Lett] 1983 Jun 27; Vol. 157 (1), pp. 210-4. - Publication Year :
- 1983
-
Abstract
- The dimeric tryptophanyl-tRNA synthetase from beef pancreas has been found to activate 2 tryptophans/mol enzyme [Eur. J. Biochem. (1982) 128, 389-398]. By using quenched-flow and stopped-flow methods under presteady-state conditions, we show that only one enzyme subunit operates at a time in the aminoacylation of tRNATrp and that the transfer reaction is not the rate-limiting step in the overall aminoacylation process.
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 157
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 6552979
- Full Text :
- https://doi.org/10.1016/0014-5793(83)81147-8