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Cloning and sequence analysis of the cDNA for the rat atrial natriuretic factor precursor.
- Source :
-
Nature [Nature] 1984 Jun 21-27; Vol. 309 (5970), pp. 719-22. - Publication Year :
- 1984
-
Abstract
- Atrial extracts contain factors which induce potent natriuresis changes in renal haemodynamics, and relax pre-contracted vascular smooth muscle. Low-molecular-weight peptides which mimic these actions have now been purified by several groups, including ours (see accompanying paper), and higher-molecular-weight proteins with similar but less potent biological activities have also been identified and are presumed to be precursors. If released into the circulation, these peptides, collectively called atrial natriuretic factor (ANF), may play a significant part in blood-pressure homeostasis, regulation of extracellular fluid volume and as antagonists to the hypertensive effects of the renin-angiotensin system and other hormonal and neurotransmitter systems. We describe here the isolation and characterization of rat atrial cDNA clones which encode ANF. Nucleotide sequence analysis shows that auriculin corresponds to the 25 amino acids located close to the C-terminus of a 152-amino acid ANF precursor. Analysis of the in vitro translation products of precursor ANF mRNA suggests that multiple forms of the precursor may exist.
- Subjects :
- Amino Acid Sequence
Animals
Atrial Function
Atrial Natriuretic Factor
Base Sequence
Molecular Weight
Muscle Proteins isolation & purification
Nucleic Acid Hybridization
Protein Biosynthesis
Protein Precursors isolation & purification
RNA, Messenger genetics
Rats
Cloning, Molecular
DNA analysis
Genes
Heart physiology
Muscle Proteins genetics
Natriuresis
Protein Precursors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0028-0836
- Volume :
- 309
- Issue :
- 5970
- Database :
- MEDLINE
- Journal :
- Nature
- Publication Type :
- Academic Journal
- Accession number :
- 6547210
- Full Text :
- https://doi.org/10.1038/309719a0