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Growth-stimulative effect of estrogen on androgen-dependent Shionogi carcinoma 115.
- Source :
-
Cancer research [Cancer Res] 1984 Dec; Vol. 44 (12 Pt 1), pp. 5644-9. - Publication Year :
- 1984
-
Abstract
- The stimulative effect of 17 beta-estradiol on the growth of androgen-dependent Shionogi carcinoma 115 and estrogen receptor in the tumor were studied. The incorporation of 17 beta-[3H]estradiol following a single injection of 17 beta-[3H]estradiol into tumor-bearing animals was 5- to 20-fold higher in the tumor than in the spleen and blood. Scatchard plot analyses showed that the tumor cytosol possessed a 17 beta-estradiol-binding site having a high affinity for 17 beta-estradiol [Kd 1.1 +/- 0.1 nM (S.E.)]. Competition experiments demonstrated that the 17 beta-estradiol binding was specific only for estrogenic compounds. Using sedimentation coefficient obtained by high-salt sucrose gradient (4.0S) and Stokes radius obtained by gel chromatography on Sephadex G-200 (46 A), the molecular weight of 17 beta-estradiol-binding component in the tumor cytosol was estimated to be 76,400. In castrated DS mice, a slight but significant increase in growth of s.c. grafted tumors was found by daily s.c. injections of either 17 beta-estradiol or 10 micrograms per mouse of testosterone propionate. Growth of the tumor maintained by 10 micrograms of testosterone propionate was augmented markedly by the addition of 4 micrograms of 17 beta-estradiol, and the growth approached the level induced by 100 micrograms of testosterone propionate. Simultaneous injections of bromocryptine inhibited an increase in 17 beta-estradiol-induced prolactin secretion but had no effect on the 17 beta-estradiol-enhanced tumor growth. These results demonstrate for the first time the stimulative effect of estrogen on the growth of androgen-dependent Shionogi carcinoma 115. The tumor contains typical estrogen receptor, which might be able to transmit estrogen signal to tumor cell nuclei with regard to tumor growth.
- Subjects :
- Animals
Castration
Cell Division drug effects
Cell Line
Cytosol metabolism
Estradiol metabolism
Male
Mammary Neoplasms, Experimental metabolism
Mice
Mice, Inbred Strains
Receptors, Androgen analysis
Receptors, Estrogen analysis
Spleen metabolism
Androgens physiology
Estradiol pharmacology
Mammary Neoplasms, Experimental pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 44
- Issue :
- 12 Pt 1
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 6498825