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Radiochemical synthesis of DL-canaline and the colorimetric assay of canaline.
Radiochemical synthesis of DL-canaline and the colorimetric assay of canaline.
- Source :
-
Analytical biochemistry [Anal Biochem] 1984 Jul; Vol. 140 (1), pp. 246-9. - Publication Year :
- 1984
-
Abstract
- A procedure is available for the production of DL-[carboxy-14C]canaline from [14C]cyanide by reaction of ethyl N-hydroxyacetimidate and acrolein to form ethyl N-[3-oxopropoxy]acetimidate. The reaction product is converted to the nitrile and then to the hydantoin derivative of DL-canaline; alkaline hydrolysis produces the free amino acid (2-amino-4-aminooxypropionic acid). This procedure can be extended to the production of DL-[carboxy-14C]canavanine by guanidination of C-1-labeled DL-canaline with O-methylisourea. A markedly improved colorimetric assay for canaline has been achieved by a procedure involving carbamylation of canaline with cyanate to form O-ureidohomoserine (2-amino-4-ureidooxybutyric acid). Colorimetric analysis of the latter amino acid markedly enhances the sensitivity, reproducibility, and accuracy of the analysis of L-canaline from biological materials.
Details
- Language :
- English
- ISSN :
- 0003-2697
- Volume :
- 140
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6486410
- Full Text :
- https://doi.org/10.1016/0003-2697(84)90160-x