Back to Search Start Over

Radiochemical synthesis of DL-canaline and the colorimetric assay of canaline.

Radiochemical synthesis of DL-canaline and the colorimetric assay of canaline.

Authors :
Rosenthal GA
Thomas D
Source :
Analytical biochemistry [Anal Biochem] 1984 Jul; Vol. 140 (1), pp. 246-9.
Publication Year :
1984

Abstract

A procedure is available for the production of DL-[carboxy-14C]canaline from [14C]cyanide by reaction of ethyl N-hydroxyacetimidate and acrolein to form ethyl N-[3-oxopropoxy]acetimidate. The reaction product is converted to the nitrile and then to the hydantoin derivative of DL-canaline; alkaline hydrolysis produces the free amino acid (2-amino-4-aminooxypropionic acid). This procedure can be extended to the production of DL-[carboxy-14C]canavanine by guanidination of C-1-labeled DL-canaline with O-methylisourea. A markedly improved colorimetric assay for canaline has been achieved by a procedure involving carbamylation of canaline with cyanate to form O-ureidohomoserine (2-amino-4-ureidooxybutyric acid). Colorimetric analysis of the latter amino acid markedly enhances the sensitivity, reproducibility, and accuracy of the analysis of L-canaline from biological materials.

Details

Language :
English
ISSN :
0003-2697
Volume :
140
Issue :
1
Database :
MEDLINE
Journal :
Analytical biochemistry
Publication Type :
Academic Journal
Accession number :
6486410
Full Text :
https://doi.org/10.1016/0003-2697(84)90160-x