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Effect of isoproterenol on protein phosphorylation in myocardial ischaemia.
- Source :
-
General physiology and biophysics [Gen Physiol Biophys] 1984 Jun; Vol. 3 (3), pp. 193-9. - Publication Year :
- 1984
-
Abstract
- Perfused rat hearts prelabelled with 32P were made ischaemic by reducing the medium flow from 12 ml/min to 0.5 ml/min. There was a rapid decrease in the contractile performance, but no significant changes in the phosphorylation state of troponin I, myosin P-light chain, an 11 K protein or in the proportion of phosphorylase in the a form occurring up to 5 min of ischaemia. Control hearts stimulated with a bolus of isoproterenol showed a large increase in the contractile force and in the phosphorylation of troponin I, 11 K protein, and phosphorylase, respectively. These responses were progressively reduced by increasing periods of ischaemia. The reduction and loss of increased phosphorylation of these proteins on exposure to isoproterenol was parallelled with an inhibition of cyclic AMP accumulation in the ischaemic heart. Phosphorylation of the myosin P-light chain remained unchanged under all the conditions studied.
- Subjects :
- Animals
Calcium-Binding Proteins metabolism
Heart drug effects
Kinetics
Myocardial Contraction drug effects
Phosphorus Radioisotopes
Phosphorylases metabolism
Phosphorylation
Rats
Rats, Inbred Strains
Troponin metabolism
Troponin I
Coronary Disease metabolism
Heart physiopathology
Isoproterenol pharmacology
Myocardium metabolism
Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0231-5882
- Volume :
- 3
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- General physiology and biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 6479578