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Characterization of a soluble suppressor of human B cell immunoglobulin biosynthesis produced by a continuous human suppressor T cell line.
- Source :
-
The Journal of experimental medicine [J Exp Med] 1981 Jul 01; Vol. 154 (1), pp. 156-67. - Publication Year :
- 1981
-
Abstract
- A human suppressor T cell maintained in long-term culture with conditioned medium containing interleukin 2 elaborates a suppressor factor(s) that specifically inhibits human polyclonal B cell immunoglobulin biosynthesis. This soluble immune suppressor supernate of immunoglobulin production (CTC-SISS-B) shares a number of features with the previously described suppressive mediator elaborated by concanavalin A-activated human peripheral T cells (SISS-B) including: (a) the inhibition by a noncytotoxic mechanism, (b) the suppression of immunoglobulin biosynthesis either through direct action on the B cell or indirect action via the monocyte, (c) the loss of inhibition in the presence of the monosaccharide L-rhamnose, (d) the elaboration by cells irradiated with 500 ro 2,000 rad, and (e) molecular weights of 60,000--90,000. Furthermore, the suppression by this mediator appears to be specific for B cell immunoglobulin production in that CTC-SISS B has no effect on T cell proliferation to mitogens, antigens, an allogeneic cells or on T cell-mediated cytotoxicity. These data indicate that one possible mechanism of suppressor T cell inhibition of human immunoglobulin production is via the generation of a lectinlike suppressor lymphokine that interacts with defined saccharide determinants on the cell surface of either the B cell or monocyte.
- Subjects :
- Animals
Antibodies
Antibodies, Monoclonal
Chromatography, Gel
Culture Media
Cytotoxicity, Immunologic
Fucose pharmacology
Galactose pharmacology
Humans
Kinetics
Phytohemagglutinins pharmacology
Rabbits
Rhamnose pharmacology
Solubility
Thymidine metabolism
Time Factors
B-Lymphocytes immunology
Immunoglobulins biosynthesis
T-Lymphocytes immunology
T-Lymphocytes, Regulatory immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1007
- Volume :
- 154
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 6454754
- Full Text :
- https://doi.org/10.1084/jem.154.1.156