Cytochemical interaction of nucleolus and cytoplasm in the Purkinje cells of senile white rats under the influence of centrophenoxine.

Senile white rats were treated with centrophenoxine at a dosage of 100 mg/Kg body weight/day for 60 days intraperitoneally. Sections of variously fixed and embedded cerebella were studied cytochemically to note the effect of the drug on the senile Purkinje neurons. The nucleolus was found to be hyper-active, as evidenced by the processes of budding and extrusion. A frank regeneration of the Nissl patches along with an increase in alpha-esterase and decrease in the activity of acid phosphatase and simple esterase was noted in the Purkinje cells after 60 days' treatment. It is suggested that the drug exerts its positive effects by regenerating the general cytoplasm and by revitalizing the nucleocytoplasmic interactions in the senile Purkinje cells.