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Gamma heavy chain disease studied by two-dimensional electrophoresis and immuno-blotting techniques.
- Source :
-
Clinical chemistry [Clin Chem] 1984 Dec; Vol. 30 (12 Pt 1), pp. 2021-5. - Publication Year :
- 1984
-
Abstract
- We used two-dimensional polyacrylamide gel electrophoresis and immunoblotting techniques to study serum proteins from a patient with a monoclonal gammopathy. Two-dimensional electrophoresis was optimized for serum proteins with two main goals: (a) to allow the resolution of many serum proteins in both directions, with penetration of the maximum number of proteins in the first dimension; and (b) to obtain the best reproducibility from one experiment to another, within the limits of the current technique. These analyses, combined with immunoblotting, permitted us to characterize a gamma heavy chain disease protein of 34 000-Da molecular mass. Moreover, two-dimensional mapping of the patient's serum proteins allowed demonstration of the microheterogeneity of this monoclonal component.
- Subjects :
- Electrophoresis, Cellulose Acetate
Electrophoresis, Polyacrylamide Gel
Humans
Immunodiffusion
Immunoelectrophoresis
Immunoenzyme Techniques
Isoelectric Focusing
Molecular Weight
Blood Proteins analysis
Electrophoresis
Heavy Chain Disease blood
Immunoglobulin Heavy Chains analysis
Immunoglobulin gamma-Chains analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9147
- Volume :
- 30
- Issue :
- 12 Pt 1
- Database :
- MEDLINE
- Journal :
- Clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6437695