Histochemical aspects concerning the synthesis and the fate of cholesterol into the epidermis.

By means of a suitable histochemical method free cholesterol and its esters could be detected into the epidermis layers. The results show that in the stratum spinosum keratinocytes free cholesterol appears as an amorphous or granular structure apparently protein unbound; into the stratum granulosum keratinocytes the cholesterol becomes protein-bound and its most part undergoes esterified. The extracellular compartment nearly the stratum granulosum contains a little amount of cholesterol esters loosely bound to proteins. The results suggest that free cholesterol after being synthesized into the cytoplasm of the stratum spinosum and granulosum keratinocytes, it is partially esterified and becomes protein-bound, appearing as fine granules within the cytoplasm of the granulous cells. From this site it takes to fates:1. Its most part remains into the granulous cell cytoplasm and at the same time the granulous cell develop to the horny cell it is placed on the thick cell membrane inner surface contributing to its thickness; 2. Another part after reaching the extracellular compartment it is spread over the thick membrane out surface. Inside the thick cell membrane, into the horny layer, free cholesterol is continuously esterified since the keratinizing cell migrate to the periphery; however even at the most peripheral layers the free cholesterol predominates. Either free cholesterol or its esters, contained into the keratinizing cell thick membrane, were excreted throughout the horny layer exfoliation. The keratinizing cell cytoplasm does not contain neither free cholesterol nor its esters.