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Isolation and characterization of large (0.5 - 1.0 micron) cytoskeleton-free vesicles from human and rabbit erythrocytes.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1983 Mar 09; Vol. 728 (3), pp. 383-93. - Publication Year :
- 1983
-
Abstract
- Large (0.5 - 1.0 micron) cytoskeleton-free vesicles were obtained, by 'budding', from fresh human and rabbit erythrocytes incubated at 45 degrees C and titrated with EDTA and CaCl2. This process occurs without hemolysis. The isolated vesicles maintain their cytoplasmic integrity and normal membrane orientation, and are resistant to hemolysis over the pH range 5.0 - 11.0 and temperature range 4-50 degrees C. The only membrane proteins detected in vesicles from human erythrocytes were band 3 region polypeptides and bands PAS-1, PAS-2 and PAS-3. Vesicles obtained from rabbit erythrocytes were similarly simple. Because of their size and stability these vesicles are amenable to both kinetic and quantitative analysis using the same experimental techniques employed in studies of synthetic lipid membranes. The results obtained in this study indicate that these vesicles are essentially markedly simplified biological cells, and thus may be useful as a biologically relevant model membrane system for examining the molecular interactions which occur within, across and between cell membranes.
- Subjects :
- Animals
Calcium Chloride pharmacology
Edetic Acid pharmacology
Erythrocyte Membrane drug effects
Freeze Fracturing
Hemolysis
Humans
Membrane Proteins isolation & purification
Microscopy, Electron
Rabbits
Species Specificity
Erythrocyte Membrane ultrastructure
Erythrocytes ultrastructure
Membrane Proteins blood
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 728
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 6402012
- Full Text :
- https://doi.org/10.1016/0005-2736(83)90510-2