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On the origin of chromosomal aberrations in human peripheral lymphocytes in vitro. I. Experiments with Neurospora endonuclease and polyethylene glycol.
- Source :
-
Human genetics [Hum Genet] 1984; Vol. 66 (4), pp. 335-43. - Publication Year :
- 1984
-
Abstract
- Post-treatment of mutagen-treated human peripheral lymphocytes with a single-strand specific endonuclease from Neurospora crassa leads to a significant elevation of the rate of structural chromosomal aberrations. Our results indicate that DNA double-strand breaks (DSB) are ultimate lesions for the formation of chromosomal aberrations in the G1 and G2 phase of the cell cycle and probably also in the S-phase. Post-treatment of X-irradiated G2 cells with polyethylene glycol (PEG) leads to an elevation of the frequencies of chromatid type aberrations. This result is taken as an indication that nucleases from PEG-damaged lysosomes transform lesions in X-ray damaged chromosomes to DSB. With respect to the origin of chromosomal aberrations, our results are in favour of the breakage and reunion hypothesis of K. Sax , and not of Revell 's exchange hypothesis.
- Subjects :
- Cell Cycle
Cells, Cultured
Chromosome Aberrations radiation effects
Epoxy Compounds pharmacology
Humans
Lymphocytes drug effects
Lymphocytes radiation effects
Lymphocytes ultrastructure
Models, Genetic
Neurospora crassa enzymology
Triaziquone pharmacology
Chromosome Aberrations drug effects
Endonucleases pharmacology
Polyethylene Glycols pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0340-6717
- Volume :
- 66
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Human genetics
- Publication Type :
- Academic Journal
- Accession number :
- 6327498
- Full Text :
- https://doi.org/10.1007/BF00287637