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Action of nitroguanidinated luteinizing hormone on different rat gonadal tissues.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 1983 Dec; Vol. 33 (2-3), pp. 147-55. - Publication Year :
- 1983
-
Abstract
- The biological activities of nitroguanidinated derivatives prepared from ovine or porcine luteinizing hormone were investigated using rat Leydig cells and pseudopregnant rat ovaries. In these tissues nitroguanidyl ovine luteinizing hormone (NGoLH) or nitroguanidyl porcine luteinizing hormone (NGpLH) were unable to stimulate adenylate cyclase or steroidogenesis but were able to inhibit the binding of ovine or porcine native LH to their specific receptors. When added to incubations of isolated Leydig cells or pseudopregnant ovary slices, NGoLH as well as NGpLH inhibited the stimulating action of native LH on adenylate cyclase or steroidogenesis. However, these derivatives had no inhibiting action on the stimulation of adenylate cyclase and steroidogenesis induced in the Leydig cells by choleratoxin or on the stimulation of testosterone production induced by 8-bromo-cyclic AMP. Since NGpLH (which does not contain lysine residues or free alpha-amino groups in the beta-subunit) exhibits the same antagonist action as NGoLH, we conclude that the nitroguanidination of the alpha-subunit is sufficient to endow the derivative with antihormone properties.
- Subjects :
- Adenylyl Cyclases metabolism
Animals
Cyclic AMP metabolism
Female
Luteinizing Hormone antagonists & inhibitors
Luteinizing Hormone pharmacology
Male
Progesterone biosynthesis
Rats
Rats, Inbred Strains
Sheep
Swine
Testosterone biosynthesis
Leydig Cells drug effects
Luteinizing Hormone analogs & derivatives
Ovary drug effects
Pseudopregnancy metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0303-7207
- Volume :
- 33
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 6317482
- Full Text :
- https://doi.org/10.1016/0303-7207(83)90163-6