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Efficient isolation of endogenous rhesus retrovirus from trophoblast.
- Source :
-
Virology [Virology] 1983 Jul 30; Vol. 128 (2), pp. 518-23. - Publication Year :
- 1983
-
Abstract
- An examination of various rhesus monkey (Macaca mulatta) organs has shown a preference for type C viral antigen expression in the placenta. Separate cocultivations of isolated trophoblasts from 10 rhesus monkey placentas with cell lines from heterologous mammalian species led to rapid isolation of type C rhesus retrovirus in 4 of 10 cases. These four retrovirus isolates have been designated MMC-2 through MMC-5. Five of the remaining six sets of cocultivations grew simian foamy virus and were discontinued. Distinction of these viral isolates from the initial rhesus isolate (MMC-1) and the previous isolate from the stumptail monkey, Macaca arctoides (MAC-1), could be made by liquid DNA hybridization, although not by limited restriction endonuclease digestion. Both MAC-1 and MMC-1 were obtained in single long-term cocultivation experiments (over 7 months). The present isolates MMC-2 through MMC-5 were detected in 2 to 5 weeks. Consequently, primary trophoblast cells represent a useful differentiated cell type for isolation of infectious retrovirus from this primate species.
- Subjects :
- Animals
Cats
Cell Fusion
Cell Line
Cytopathogenic Effect, Viral
Dogs
Female
Genes, Viral
Humans
Nucleic Acid Hybridization
Pregnancy
Retroviridae genetics
Retroviridae growth & development
Virus Cultivation
Macaca microbiology
Macaca mulatta microbiology
Retroviridae isolation & purification
Trophoblasts microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0042-6822
- Volume :
- 128
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 6310872
- Full Text :
- https://doi.org/10.1016/0042-6822(83)90281-7