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Epstein-Barr virus-induced immunoglobulin synthesis by B cells from individuals with late-onset panhypogammaglobulinemia.
- Source :
-
Journal of clinical immunology [J Clin Immunol] 1983 Jul; Vol. 3 (3), pp. 253-9. - Publication Year :
- 1983
-
Abstract
- Epstein-Barr virus (EBV) transformation was used to examine the differentiation potential of circulating B cells from eight individuals with late-onset panhypogammaglobulinemia. Cytoplasmic and secreted immunoglobulins were evaluated by immunofluorescence and radioimmunoassay. EBV-infected cultures of B cells from patients and healthy controls generated similar numbers of IgM-secreting plasma cells, but relatively few IgG and IgA plasma cells were induced in cultures of patients' B cells. As further evidence of B-cell immaturity, approximately 90% of the IgA B cells in the eight patients coexpressed IgM. Clonal diversity of B cells from hypogammaglobulinemic patients was examined with a panel of mouse monoclonal antibodies directed against idiotypic and VH subgroup determinants. The frequencies of EBV-induced plasma cells exhibiting the different idiotypic and VH determinants were similar for patients and controls. The data suggest the continued generation of clonally diverse B cells that are capable of terminal plasma-cell differentiation when the normal triggering mechanisms are bypassed by EBV. The arrested differentiation at an immature B-cell stage in these hypogammaglobulinemic individuals would appear to reflect a defect in normal B-cell triggering.
- Subjects :
- Adult
Antibodies, Monoclonal immunology
Antibody Diversity
Clone Cells immunology
Female
Humans
Immunoglobulin Heavy Chains immunology
Immunoglobulin Idiotypes immunology
Immunoglobulin M metabolism
Male
Middle Aged
Plasma Cells microbiology
Agammaglobulinemia blood
B-Lymphocytes metabolism
Herpesvirus 4, Human
Immunoglobulins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0271-9142
- Volume :
- 3
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 6309895
- Full Text :
- https://doi.org/10.1007/BF00915349