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Benzo(a)pyrene phenol production by perfused rat liver and its inhibition by ethanol.
- Source :
-
Cancer research [Cancer Res] 1982 May; Vol. 42 (5), pp. 1681-5. - Publication Year :
- 1982
-
Abstract
- A rapid and inexpensive method has been developed to estimate rates of benzo(a)pyrene phenol production by perfused rat liver. This method is based on the measurement of benzo(a)pyrene phenols utilizing a simple fluorometric procedure. Within 2 to 3 min after infusion of benzo(a)pyrene bound to serum albumin, phenols are excreted into the perfusate, primarily as glucuronide and sulfate conjugates. Maximal rates of phenol release were 8 to 10 nmol/g/hr in livers from control rats and 40 to 42 nmol/g/hr in livers from 3-methylcholanthrene-treated rats. Fasting of 3-methylcholanthrene-treated rats for 24 hr prior to perfusion experiments did not affect either the rate of phenol production or the extent of their conjugation. Ethanol (20 mM) inhibited rates of phenol formation by 50% in livers from fasted, 3-methylcholanthrene-treated rats but had no effect on benzo(a)pyrene hydroxylase activity in isolated hepatic microsomes. These data indicate that ethanol inhibits phenol formation from benzo(a)pyrene in intact liver, probably by diminishing the supply of the cofactor reduced nicotinamide adenine dinucleotide phosphate.
- Subjects :
- Animals
Benzo(a)pyrene
Benzopyrenes administration & dosage
Depression, Chemical
Female
Hydroxylation
Liver drug effects
Methylcholanthrene administration & dosage
Methylcholanthrene metabolism
Microsomes, Liver metabolism
Perfusion
Rats
Rats, Inbred Strains
Time Factors
Benzopyrenes metabolism
Ethanol pharmacology
Liver metabolism
Phenols metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 42
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 6279282