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Effects of superoxide dismutase on cholesterol 7 alpha-hydroxylation in swine.
- Source :
-
Steroids [Steroids] 1980 Apr; Vol. 35 (4), pp. 459-69. - Publication Year :
- 1980
-
Abstract
- Effects of carbon monoxide, nitrogen, ferricytochrome c and p-hydroxymercuribenzoate were studied on cholesterol 7 alpha-hydroxylase activity of swine hepatic microsomes. The results suggest that a microsomal electron transport system is involved in hepatic microsomal cholesterol 7 alpha-hydroxylation in swine. Cholesterol 7 alpha-hydroxylation is inhibited by superoxide dismutase in the standard assay system containing a NADPH generating system. Superoxide dismutase also inhibited cholesterol 7 alpha-hydroxylation in the system where superoxides were generated by enzymatic or nonenzymatic means in the absence of NADPH-generating system. The current study suggests that superoxide anion may be an important factor in the cholesterol 7 alpha-hydroxylation of swine.
- Subjects :
- Animals
Carbon Monoxide pharmacology
Cytochrome c Group pharmacology
Hydroxymercuribenzoates pharmacology
Hypoxia
Male
Swine
Cholesterol 7-alpha-Hydroxylase antagonists & inhibitors
Microsomes, Liver enzymology
Steroid Hydroxylases antagonists & inhibitors
Superoxide Dismutase pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0039-128X
- Volume :
- 35
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Steroids
- Publication Type :
- Academic Journal
- Accession number :
- 6246663
- Full Text :
- https://doi.org/10.1016/0039-128x(80)90146-4