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[Determination of acetylcholine by inhibition of the specific binding of tritiated cis-methyldioxolane: application to the study of the effects of acetylcholine accumulation in a murine neuroblastoma culture].

Authors :
Blanchet G
Lallement G
Baubichon D
Mavet S
Source :
Comptes rendus des seances de la Societe de biologie et de ses filiales [C R Seances Soc Biol Fil] 1984; Vol. 178 (5), pp. 526-34.
Publication Year :
1984

Abstract

The radioreceptor assay for acetylcholine (ACh) is based on the ability of the ACh to compete with the specific binding of [3H] cis-methyldioxolane to muscarinic receptors of rat cerebral cortex membranes. The technique described was used to measure ACh levels in NS 20 cells treated with an organophosphorus compound. The down regulation of muscarinic acetylcholine receptors of neuroblastoma cells observed is probably related to ACh accumulation.

Details

Language :
French
ISSN :
0037-9026
Volume :
178
Issue :
5
Database :
MEDLINE
Journal :
Comptes rendus des seances de la Societe de biologie et de ses filiales
Publication Type :
Academic Journal
Accession number :
6241025