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Peptide chain initiation and analysis of in vitro translation products in rat heart undergoing hypertrophic growth.

Authors :
Mezzetti G
Ferrari S
Davalli P
Battini R
Corti A
Source :
Journal of molecular and cellular cardiology [J Mol Cell Cardiol] 1983 Sep; Vol. 15 (9), pp. 629-35.
Publication Year :
1983

Abstract

The cytosol fraction of rat heart contains an initiation factor-like protein component that behaves like the eukaryotic factor (eIF-2) in binding [35S]-Met-tRNAf in the presence of GTP. The ternary initiator complex thus formed is able to bind to heart ribosomes. In the left ventricle of rat heart undergoing hypertrophic growth upon constriction of the descending aorta, the [35S]-Met-tRNAf binding activity of the cytosol protein(s) gradually increases after the operation from 40%, at 48 h, to 90%, at 10 days; slightly lower activation is seen in the [35S]-Met-tRNAf binding to ribosomes. Polysomal RNA is extracted from sham operated and hypertrophic rat hearts and translated in a reticulocyte cell free system. The translation products are analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis: no clearcut qualitative difference is observed in the pattern obtained from sham operated and hypertrophic animals.

Details

Language :
English
ISSN :
0022-2828
Volume :
15
Issue :
9
Database :
MEDLINE
Journal :
Journal of molecular and cellular cardiology
Publication Type :
Academic Journal
Accession number :
6226806
Full Text :
https://doi.org/10.1016/0022-2828(83)90272-9