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High-performance liquid chromatography for assaying NAD glycohydrolase from Neurospora crassa conidia.
- Source :
-
Analytical biochemistry [Anal Biochem] 1983 Jun; Vol. 131 (2), pp. 533-7. - Publication Year :
- 1983
-
Abstract
- A rapid and sensitive high-performance liquid chromatographic technique was developed to determinate NAD glycohydrolase (EC 3.2.2.5.) activity from Neurospora crassa conidia. The separation of the assay substrate and products was achieved by isocratic reverse-phase chromatography and the peaks were detected by the absorbance at 259 nm. Quantities of NAD+ and nicotinamide as small as 10 pmol could be measured.
Details
- Language :
- English
- ISSN :
- 0003-2697
- Volume :
- 131
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 6225349
- Full Text :
- https://doi.org/10.1016/0003-2697(83)90211-7