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Characterization of the subpopulations of human peripheral blood B lymphocytes which react to staphylococcus aureus Cowan I and T-cell help.
- Source :
-
Cellular immunology [Cell Immunol] 1983 May; Vol. 78 (1), pp. 130-43. - Publication Year :
- 1983
-
Abstract
- Staphylococcus aureus Cowan I strain (SAC) activates human peripheral blood B lymphocytes to proliferate but does not induce secretion of immunoglobulin (Ig) unless pokeweed mitogen (PWM)-activated T cells are added. Using a Percoll gradient separation method, we show that a B-cell subpopulation isolated at 50/60% Percoll interface is the target for SAC stimulation. After SAC activation, small, dense B cells become larger, less-dense B cells which proliferate. These activated B blasts differentiate into Ig-secreting cells when cultured with PWM-activated T cells. The culture supernatant of PWM-activated T cells is capable of supporting proliferation and Ig secretion of the activated B blasts but not of resting small B cells. This result suggests that the culture supernatant contains B-cell growth factors.
- Subjects :
- Adult
Antibody-Producing Cells classification
Antibody-Producing Cells cytology
Antibody-Producing Cells immunology
B-Lymphocytes cytology
B-Lymphocytes immunology
Cell Differentiation
Cell Separation
Growth Substances physiology
Hemolytic Plaque Technique
Humans
Interleukin-4
Interleukin-5
Lymphokines physiology
Pokeweed Mitogens pharmacology
Staphylococcus aureus immunology
B-Lymphocytes classification
Lymphocyte Activation
T-Lymphocytes, Helper-Inducer immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-8749
- Volume :
- 78
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 6221807
- Full Text :
- https://doi.org/10.1016/0008-8749(83)90266-6