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Production of human suppressor T cell hybridomas.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1982 Nov; Vol. 129 (5), pp. 1986-92. - Publication Year :
- 1982
-
Abstract
- To study human T cell suppression of immunoglobulin (Ig) synthesis with homogeneous populations of immunoregulatory cells, human suppressor T cell hybridomas were prepared by somatic cell fusion of concanavalin A-activated peripheral blood T cells with hypoxanthine-guanine phosphoribosyltransferase-(HGPRT, EC 2.4.2.8) deficient human leukemic CEM T cells. After selection in hypoxanthine-aminopterin-thymidine (HAT) medium and cloning by limiting cell dilution, two human T cell hybridomas were identified that produced 60 to 80% suppression of in vitro polyclonal immunoglobulin production when cocultured with pokeweed mitogen- (PWM) stimulated peripheral blood lymphocytes. Further, one of the suppressor T cell hybridomas constitutively secreted a soluble suppressor factor(s) (TsF) of m.w. 70,000 to 85,000 daltons, which produced reversible noncytotoxic inhibition of lectin-activated B cell Ig production. In contrast, this TsF did not inhibit lectin- or antigen-induced T cell proliferation, nor did it interfere with the generation or effector function of cytotoxic T cells. Additional studies indicated that this Tsf acts directly on B cells or monocytes rather than indirectly modulating the activity of immunoregulatory T cells. In summary, these studies suggest that techniques of somatic cell fusion may provide a valuable approach to further study human immunoregulatory cell-cell interactions as well as provide a source of sufficient quantities of important lymphokines for further purification and characterization.
- Subjects :
- Cell Division
Clone Cells cytology
Clone Cells immunology
Humans
Hybridomas cytology
Immunoglobulins biosynthesis
Lymphokines analysis
Lymphokines biosynthesis
Phenotype
Pokeweed Mitogens pharmacology
Suppressor Factors, Immunologic
T-Lymphocytes cytology
T-Lymphocytes immunology
T-Lymphocytes, Regulatory immunology
Cell Fusion
Hybridomas immunology
Immunologic Techniques
T-Lymphocytes, Regulatory cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 129
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 6214585