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Enrichment of epidermal Langerhans cells: studies with a monolayer technique and flow cytometry sorting.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 1982 Jun; Vol. 78 (6), pp. 452-5. - Publication Year :
- 1982
-
Abstract
- Langerhans cells were enriched from epidermal cell suspensions by a monolayer technique based on the association of Langerhans cells with solid matrix-bound anti-Ia antibodies or by flow cytometry sorting of fluorescein-isothiocyanate labeled anti-Ia reactive cells. The monolayer technique gave a moderate enrichment (15-37%) whereas considerably higher purity (73-87%) was obtained by flow cytometry sorting. The identity of the enriched anti-Ia reactive cells as Langerhans cells was established by histochemical techniques or electron microscopy. The monolayer-enriched Langerhans cells could function as stimulating cells in the mixed leukocyte culture reaction and as antigen-presenting cells.
Details
- Language :
- English
- ISSN :
- 0022-202X
- Volume :
- 78
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 6211496
- Full Text :
- https://doi.org/10.1111/1523-1747.ep12510125