Back to Search
Start Over
Cytokeratin and neurofilament in lung carcinomas.
- Source :
-
The American journal of pathology [Am J Pathol] 1984 Sep; Vol. 116 (3), pp. 363-9. - Publication Year :
- 1984
-
Abstract
- Three monoclonal antibodies, one directed against cytokeratin (clone 80) and two directed against neurofilament (clones 2F11 and 3G6), were used in the study of a series of 77 lung carcinomas by immunohistochemical staining. The anti-cytokeratin antibody, a very broadly reacting antibody directed against an antigenic determinant common to a great number of cytokeratins, was applicable on frozen sections. The two anti-neurofilament antibodies, directed against the 70 kD protein (clone 2F11) and the 160 kD and 200 kD proteins (clone 3G6) of neurofilament, were applicable on both frozen sections and paraffin sections. The staining results on the lung carcinomas indicate that all types of tumors studied, including small-cell anaplastic carcinoma, are markedly positive for cytokeratin. Frozen sections of five and formalin-fixed and paraffin-embedded sections of six other small-cell anaplastic carcinomas were negative with both anti-neurofilament monoclonal antibodies. One poorly differentiated squamous cell carcinoma positive with anti-neurofilament clone 2F11 but negative with clone 3G6. This distribution of cytoskeletal proteins demonstrates the epithelial differentiation of all types of lung carcinomas. Neuroendocrine differentiation of lung carcinomas as found in the small-cell anaplastic types does not result in expression of neurofilament proteins.
- Subjects :
- Adenocarcinoma metabolism
Adenocarcinoma, Bronchiolo-Alveolar metabolism
Antibodies, Monoclonal
Carcinoma, Small Cell metabolism
Carcinoma, Squamous Cell metabolism
Histocytochemistry
Humans
Neurofilament Proteins
Intermediate Filament Proteins metabolism
Keratins metabolism
Lung Neoplasms metabolism
Neoplasm Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9440
- Volume :
- 116
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The American journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 6206727